Evaluation of a flow cytofluorometric method for rapid determination of amphotericin B susceptibility of yeast isolates.

نویسندگان

  • F Peyron
  • A Favel
  • H Guiraud-Dauriac
  • M El Mzibri
  • C Chastin
  • G Dumenil
  • P Regli
چکیده

A rapid-flow cytofluorometric susceptibility test for in vitro amphotericin B testing of yeasts was evaluated and compared to the National Committee for Clinical Laboratory Standards (NCCLS) M27-T reference broth macrodilution method. The flow cytofluorometric method is based on the detection of decreased green fluorescence intensity of cells stained with DiOC5(3), a membrane potential-sensitive cationic dye, after drug treatment. Testing was performed on 134 clinical isolates (Candida spp. and Torulopsis glabrata). From the dose-response curve obtained for each isolate, three endpoints were calculated by computer analysis (the concentrations at which the fluorescence intensity was reduced by 50, 80, and 90%, i.e., 50% inhibitory concentration [IC50], IC80, and IC90, respectively). A regression analysis correlating these endpoints with the M27-T MICs showed that the best agreement was obtained with IC80. The flow cytofluorometric method showed good reproducibility with control strains. These initial results suggest that the flow cytofluorometric method is a valid alternative to the NCCLS reference method.

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عنوان ژورنال:
  • Antimicrobial agents and chemotherapy

دوره 41 7  شماره 

صفحات  -

تاریخ انتشار 1997